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biotinylated polyclonal sheep anti human fap antibody  (R&D Systems)


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    R&D Systems biotinylated polyclonal sheep anti human fap antibody
    Biotinylated Polyclonal Sheep Anti Human Fap Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+polyclonal+sheep+anti+human+fap+antibody/Human+Fibroblast+Activation+Protein+alpha%2FFAP+Biotinylated+Antibody/pmc11346374-113-3-11
    Average 93 stars, based on 9 article reviews
    biotinylated polyclonal sheep anti human fap antibody - by Bioz Stars, 2026-10
    93/100 stars

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    Incubation:

    Article Title: Fibroblast activation protein is a cellular marker of fibrotic activity in canine idiopathic pulmonary fibrosis
    Article Snippet: .. For detection, a biotinylated polyclonal sheep anti-human FAP antibody (0.4 μg/μl, R&D Systems #BAF3715, RRID:AB_2057508) was added and incubated for 90 min. Plates were then incubated with avidin horseradish peroxidase (1:1000 dilution, Thermo Fisher Scientific #18–4100-94) for 30 min, after which 3,3′,5,5′-tetramethylbenzidine (TMB, Life Technologies #SB02) was added. .. After a 10 min-incubation in the dark, reaction was stopped using 50 μL/well of H2SO4 1 M. Plates were read by an optical density reader (Multiskan FC, Thermo Fisher Scientific #51119000) set at 450 nm.

    Avidin-Biotin Assay:

    Article Title: Fibroblast activation protein is a cellular marker of fibrotic activity in canine idiopathic pulmonary fibrosis
    Article Snippet: .. For detection, a biotinylated polyclonal sheep anti-human FAP antibody (0.4 μg/μl, R&D Systems #BAF3715, RRID:AB_2057508) was added and incubated for 90 min. Plates were then incubated with avidin horseradish peroxidase (1:1000 dilution, Thermo Fisher Scientific #18–4100-94) for 30 min, after which 3,3′,5,5′-tetramethylbenzidine (TMB, Life Technologies #SB02) was added. .. After a 10 min-incubation in the dark, reaction was stopped using 50 μL/well of H2SO4 1 M. Plates were read by an optical density reader (Multiskan FC, Thermo Fisher Scientific #51119000) set at 450 nm.

    Control:

    Article Title: Fibroblast activation protein augments progression and metastasis of pancreatic ductal adenocarcinoma.
    Article Snippet: Cells were incubated with Fc receptor blocker (eBioscience, catalog 14-0161-85) followed by staining with the following fluorescence-conjugated antibodies: PE/Cy7 anti-mouse CD45 (catalog 103114), PE anti-mouse CD90.2 (catalog 140308), and APC Streptavidin (catalog 405207) (Biolegend). .. Biotinylated polyclonal sheep anti-human FAP antibody that cross-reacts with mouse FAP (catalog BAF3715) and biotinylated normal sheep IgG control (catalog BAF020) were purchased from R&D Systems. ..



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